Coding
cheZ

Part:BBa_K629003

Designed by: Zilong WANG, Yi ZHENG   Group: iGEM11_SYSU-China   (2011-10-03)
Revision as of 02:17, 6 October 2011 by Skybird (Talk | contribs) (→‎Expression in E. Coli)

cheZ, chemotaxis regulator, protein phosphatase for CheY


Background

Polar localization is dependent on CheA-short. CheZ is a cytosolic phosphatase which functions in the chemotaxis signal transduction complex. Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).


Expression in E. Coli

Before we started our functional test of CheZ, we have utilized western blot to determine whether there are enough soluble proteins in E. Coli. With 6×His tag on pET-32a, the result shows that CheZ has more expression with induction of IPTG.

Note: Because the promoter is Plac here, the expression has a background, which means control also expresses some cheZ without induction of IPTG.

CheZ.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/phosphorylation
//chassis/prokaryote/ecoli
//function/motility
Parameters
functiondephosphorylate CheY to move E. Coli forward
n/acheZ, chemotaxis regulator, protein phosphatase for CheY
origingenome of E. Coli BL21 Plys