Device

Part:BBa_K658003:Design

Designed by: Zhizhen Zhao   Group: iGEM11_XMU-China   (2011-09-29)
Revision as of 02:14, 6 October 2011 by Xmuwendy (Talk | contribs) (→‎Design Notes)

a bacteria population-control device with RBS0.6 driven by lacl+pL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2347
    Illegal BsaI.rc site found at 1874


Design Notes

Its design consideration is the same as BBa_K658001

The part was designed to combine three parts together to construct a population-control device.The three subparts are a LuxI producer, a LuxR producer and a killer protein producer. 1) LuxI producer 2) LuxR producer

XXXXXXXXXXX FIG.1 XXXXXXXXXXXXXXX

3)killer protein producer

XXXXXXXXXXX FIG.2 XXXXXXXXXXXXXXX

The device is successfully constructed which can be verified by the restriction analysis shown as follows:

XXXXXXXXXXX FIG.3 XXXXXXXXXXXXXXX

Source

References