Regulatory
praiI

Part:BBa_K594009:Design

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-10-01)
Revision as of 00:28, 6 October 2011 by DonQuixia (Talk | contribs) (→‎Design Notes)

the promoter of raiI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 444
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 447


Design Notes

The Rhizobium etli CFN42 is also a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in Rhizobium etli CFN42, and then found the specific sequence in NCBI and asked the Rhizobium etli CFN42 from Nanjing Agricultural University. The raiI/R system have no threats to biosafety.

Source

The promoter of raiI is from the Rhizobium etli CFN42.

References