Coding
raiI

Part:BBa_K594007:Design

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-10-01)
Revision as of 00:14, 6 October 2011 by DonQuixia (Talk | contribs) (→‎Design Notes)

a luxI homologous AHL synthetase from Rhzobium etli CFN 42


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 28
    Illegal XhoI site found at 583
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 403
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 31


Design Notes

The Rhizobium etli CFN42 is also a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in Rhizobium etli CFN42, and then found the specific sequence in NCBI and asked the Rhizobium etli CFN42 from Nanjing Agricultural University. The raiI/R system have no threats to biosafety.

Source

This part is from Rhzobium etli CFN42.

References