Part:BBa_R0077:Experience

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Applications of BBa_R0077

First, we induced generator cell with 10-3M IPTG for 16 hours, then centrifuged cells and collect the supernatant. We use this supernatant as original 3OH-C14:1-HSL solution，then we diluted it to 10-7, 5×10-7, 10-6, 5×10-6, 10-5, 6×10-5, 8×10-5, 10-4, 2×10-4, 4×10-4, 7×10-4, 10-3, 3×10-3, 6×10-3, 8×10-3, 10-2 times of original solution concentration respectively. Using these diluted supernatants with different concentration of 3OH-C14:1-HSL to induce receiver cells (OD600 0.4) each at 37℃ for 3 hours, then centrifuged cells and resuspended in phosphate buffer solution (PBS). The GFP fluorescence of each culture was obtained using enzyme-labeled assay. The result is shown in figure First, we induced generator cell with 10-3M IPTG for 16 hours, then centrifuged cells and collect the supernatant. We use this supernatant as original 3OH-C14:1-HSL solution，then we diluted it to 10-7, 5×10-7, 10-6, 5×10-6, 10-5, 6×10-5, 8×10-5, 10-4, 2×10-4, 4×10-4, 7×10-4, 10-3, 3×10-3, 6×10-3, 8×10-3, 10-2 times of original solution concentration respectively. Using these diluted supernatants with different concentration of 3OH-C14:1-HSL to induce receiver cells (OD600 0.4) each at 37℃ for 3 hours, then centrifuged cells and resuspended in phosphate buffer solution (PBS). The GFP fluorescence of each culture was obtained using enzyme-labeled assay. The result is shown in figure 1.

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