Part:BBa_K594002:Design

subunit of a 3-isopropylmalate dehydrogenase from E.coli BL21

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Besides, when we were looking for leuB gene, we also try to find its RBS, but unluckily, we didn't find its RBS on NCBI, its location is closely linked to leuA's location.

Source

This leuB is from the leu operon in BL 21(E.coli),we get BL21 from Takara and found its sequence in NCBI, then cloned it with a Pfu PCR.

References

1.J. M. Somers, A. Amzallag1, and R. B. Middleton2，Genetic Fine Structure of the Leucine Operon of Escherichia coli K-12，J Bacteriol. 1973 March; 113(3): 1268-1272
2.Wallon G, Yamamoto K, Kirino H, Yamagishi A, Lovett ST, Petsko GA, Oshima T (1997). "Purification, catalytic properties and thermostability of 3-isopropylmalate dehydrogenase from Escherichia coli." Biochim Biophys Acta 1337(1);105-12. PMID: 9003442
3. Wallon G, Kryger G, Lovett ST, Oshima T, Ringe D, Petsko GA (1997). "Crystal structures of Escherichia coli and Salmonella typhimurium 3-isopropylmalate dehydrogenase and comparison with their thermophilic counterpart from Thermus thermophilus." J Mol Biol 266(5);1016-31. PMID: 9086278
4.Wright BE, Longacre A, Reimers JM (1999). "Hypermutation in derepressed operons of Escherichia coli K12." Proc Natl Acad Sci U S A 96(9);5089-94. PMID: 10220423
5. Yang HL, Kessler DP (1974). "Genetic analysis of the leucine region in Escherichia coli B-r: gene-enzyme assignments." J Bacteriol 117(1);63-72. PMID: 4587614