Part:BBa_K581003
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)
This is the conjugate part of ptsG2-gfp.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This BioBrick has been sequence verified.
Background
SgrS is an Hfq-binding small antisense RNA that is induced upon phosphosugar stress (Vanderpool, 2007). It forms a ribonucleoprotein complex with RNase E through Hfq to mediate silencing of the target ptsG mRNA encoding the major glucose transporter (Geissmann and Touati, 2004). A 31-nt-long stretch in the 3’ region of SgrS is partially complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns out to be crucial for SgrS’ function, shown as Fig 2 (Kawamoto et al., 2006; Maki et al., 2010).
Figure 1:The complementary pair site-mutant version of ptsG2 mRNA and corresponding SgrS2.
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Experimental Data
Methods
References
[1] Geissmann, T.A., and Touati, D. (2004). Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405
[2] Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61: 1013-1022
[3] Levine, E., Zhang, Z., Kuhlman, T., and Hwa, T. (2007). Quantitative characteristics of gene regulation by small RNA. PLoS biology 5: e229
//regulation/negative
| device_type | post-transcriptional regulators of gene expression |
| family | Hfq-binding small RNA |
| function | destabilzes ptsG2 mRNA |
| genotype | G176C mutant of SgrS(wt） |
| n/a | SgrS2 (conjugate part of ptsG2 in comparator) |
| origin | E.coli K-12 strain |
| target | 5' Untranslated Regions |