Regulatory
psinI
Part:BBa_K594004:Design
Designed by: Yu Xiaoyan Group: iGEM11_OUC-China (2011-10-01)
the promoter of sinI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 89
Illegal NgoMIV site found at 251 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Sinorhizobium meliloti 1021 is a mode organism in Rhizobia. We looked for the papers related to the Quorum Sensing systems in S. meliloti Rm 1021, and then found the specific sequence in NCBI and asked the S. meliloti Rm 1021 strain from Nanjing Agricultural University. The promoter of sinI have no threats to biosafety.
Source
It comes from the S. meliloti Rm1021