Part:BBa_K581003
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)
This is the conjugate part of ptsG2-gfp.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This BioBrick has been sequence verified.
Background
SgrS is an Hfq-binding small antisense RNA that is induced upon phosphosugar stress (Vanderpool, 2007). It forms a ribonucleoprotein complex with RNase E through Hfq to mediate silencing of the target ptsG mRNA encoding the major glucose transporter (Geissmann and Touati, 2004). A 31-nt-long stretch in the 3’ region of SgrS is partially complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns out to be crucial for SgrS’ function, shown as Fig 1 (Kawamoto et al., 2006; Maki et al., 2010).
[[File:M_Mechanism.png|680px]] [[File:M_P1_S1.png|680px]] [[File:M_P2_S2.png|680px]] ''Fig. 2 Sequence alignment of wild type ptsG/SgrS pair and its mutant complementary pairs.'' ''(A) The partial complementary region of ptsG (wt) mRNA and its corresponding sRNA SgrS.'' ''(B) The complementary pair of ptsG1 mRNA and corresponding SgrS1.'' ''(C) Another complementary pair site-mutant version of ptsG2 mRNA and corresponding SgrS2.''miao
Experimental Data
Methods. It forms a ribonucleoprotein complex with RNase E through Hfq to mediate silencing of the target ptsG mRNA encoding the major glucose transporter (Geissmann and Touati, 2004). A 31-nt-long stretch in the 3’ region of SgrS is partially complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns out to be crucial for SgrS’ function, shown as Fig 2 (Kawamoto et al., 2006; Maki et al., 2010).</p>
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<h2>Experimental Data</h2>
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<h3>Methods<a href=)
References
[1] Geissmann, T.A., and Touati, D. (2004). Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405
[2] Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61: 1013-1022
[3] Levine, E., Zhang, Z., Kuhlman, T., and Hwa, T. (2007). Quantitative characteristics of gene regulation by small RNA. PLoS biology 5: e229
//regulation/negative
| device_type | post-transcriptional regulators of gene expression |
| family | Hfq-binding small RNA |
| function | destabilzes ptsG2 mRNA |
| genotype | G176C mutant of SgrS(wt） |
| n/a | SgrS2 (conjugate part of ptsG2 in comparator) |
| origin | E.coli K-12 strain |
| target | 5' Untranslated Regions |