Part:BBa_K581004
ptsG2-GFP (ptsG2 5'UTR fused with gfp)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 747
This BioBrick has been sequence verified.
Background
ptsG2 is the C87G mutant of ptsG(wt) and the conjugate part of SgrS2 in our comparator device (see Fig. 1). [[Image:MM P2 S2.png]] ''Fig. 1 The complementary pair of ptsG2 mRNA and corresponding SgrS2.'' PtsG is a glucose permease which is subordinate to phosphotransferase system and serves as a transporter. Here, this mRNA performs as the conjugate part of the small RNA regulator sgrS(wt). ptsG mRNA is regulated by SgrS by short, imperfect base-pairing interactions, and its expression is thus repressed.
Experimental Data
Methods
References
[1] Geissmann, T.A., and Touati, D. (2004). Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405
[2] Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61: 1013-1022
[3] Levine, E., Zhang, Z., Kuhlman, T., and Hwa, T. (2007). Quantitative characteristics of gene regulation by small RNA. PLoS biology 5: e229
transcriptional
| biology | 5' regulatory region of ptsG2 is the target of sRNA SgrS2 |
| chassis | E. coli DH5α |
| genotype | C87G mutant of ptsG(wt) |
| n/a | ptsG2 (conjugate part of SgrS2 in comparator) |