RNA

Part:BBa_K581004

Designed by: Chen Yiwei, Qin Xiao   Group: iGEM11_Peking_S   (2011-09-26)
Revision as of 21:33, 5 October 2011 by QINXIAO (Talk | contribs)

ptsG2-GFP (ptsG2 5'UTR fused with gfp)

This is the conjugate part of the small RNA regulator sgrS2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 747
                                     Induce ptsG.png

References

[1] Geissmann, T.A., and Touati, D. (2004). Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405

[2] Kawamoto, H., Koide, Y., Morita, T., and Aiba, H. (2006). Base-pairing requirement for RNA silencing by a bacterial small RNA and acceleration of duplex formation by Hfq. Molecular microbiology 61: 1013-1022

[3] Levine, E., Zhang, Z., Kuhlman, T., and Hwa, T. (2007). Quantitative characteristics of gene regulation by small RNA. PLoS biology 5: e229

[edit]
Categories
//chassis/prokaryote/ecoli
transcriptional
Parameters
biology5' regulatory region of ptsG2 is the target of sRNA SgrS2
chassisE. coli DH5α
genotypeC87G mutant of ptsG(wt)
n/aptsG2 (conjugate part of SgrS2 in comparator)