Part:BBa_K581003

SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

This is the conjugate part of ptsG2-gfp.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

This BioBrick has been sequence verified.

Background

miao

1.2 miao[ [[Image:link.png]]width="1000px" align="right"

[[Image:Mechanism.png]]

Figure 1: Sequence alignment of wildtype ptsG/SgrS pair and its mutant complementary pairs.

References

[1] Geissmann, T.A., and Touati, D. (2004) Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. The EMBO journal 23: 396-405

[2] . :

[3] . :.

[edit]

Categories
//chassis/prokaryote/ecoli
//regulation/negative

Parameters

device_type post-transcriptional regulators of gene expression
family Hfq-binding small RNA
function destabilzes ptsG2 mRNA
genotype G176C mutant of SgrS(wt）
n/a SgrS2 (conjugate part of ptsG2 in comparator)
origin E.coli K-12 strain
target 5' Untranslated Regions

device_type	post-transcriptional regulators of gene expression
family	Hfq-binding small RNA
function	destabilzes ptsG2 mRNA
genotype	G176C mutant of SgrS(wt）
n/a	SgrS2 (conjugate part of ptsG2 in comparator)
origin	E.coli K-12 strain
target	5' Untranslated Regions