Part:BBa_K544013

tetR-HNS (2-90) fusion protein (Consitutive Promoter: BBa-J23109)

The tetR-HNS2-90 protein fuses the polymerization demain of histone-like nucleoid-structuring (H-NS) protein to tetR. Thus, in E.coli, when TetR-HNS2-90 binds the TetR-specific DNA motif, it will recruit native H-NS to bind to adjacent DNA, which may lead to local gene silencing. This fusion protein is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106). Experimental data indicates that TetR-HNS2-90 is more effective than TetR on transcription repression.

BBa_K544013 is one of the fusion protein that work as expected according to the analysis of experimental data as shown below.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 65
  Illegal SpeI site found at 37
  Illegal PstI site found at 851
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal SpeI site found at 37
  Illegal PstI site found at 851
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 65
  Illegal SpeI site found at 37
  Illegal PstI site found at 851
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 65
  Illegal SpeI site found at 37
  Illegal PstI site found at 851
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 833
  

Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors

(The data collected are considered only when difference between values with the P-value <0.05)

Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors

(The data collected are considered only when difference between values with the P-value <0.05)

[EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]