Part:BBa_K544011
tetR-HNS (2-90) fusion protein (Consitutive Promoter: BBa-J23103)
The tetR-HNS2-90 protein fuses the polymerization demain of histone-like nucleoid-structuring (H-NS) protein to tetR. Thus, in E.coli, when TetR-HNS2-90 binds the TetR-specific DNA motif, it will recruit native H-NS to bind to adjacent DNA, which may lead to local gene silencing. This fusion protein is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106). Experimental data indicates that TetR-HNS2-90 is more effective than TetR on transcription repression.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 65
Illegal SpeI site found at 37
Illegal PstI site found at 851 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 37
Illegal PstI site found at 851 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 65
Illegal SpeI site found at 37
Illegal PstI site found at 851 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 65
Illegal SpeI site found at 37
Illegal PstI site found at 851 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 833
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| Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors |
(The data collected are considered only when difference between values with the P-value <0.05)
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| Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors |
(The data collected are considered only when difference between values with the P-value <0.05) [EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]
| None |