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Composite

Part:BBa_K539281

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-17)
Revision as of 17:57, 5 October 2011 by Bettyyang (Talk | contribs)

rbs+crtZ(beta-carotene hydroxylase) under heat sensitive cI regulater

This part contains crtZ, which works as the zeaxanthin biosynthesis pathway when add beta-carotene as a substrate. crtZ is regulated by cI and cI regulated promoter. The promoter has two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. File:Pathway File:CrtZ When we increase the temperature to 42℃, the CI proteins BBa_K098997, the repressor of cI regulated promoter BBa_R0051, are fully denatured. Then the cI regulated promoter is activated and CrtZ BBa_I742158 is produced, converting the orange beta-carotene to yellow Zeaxanthin.

This biobrick can coorperate with BBa_K539151

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1492
  • 1000
    COMPATIBLE WITH RFC[1000]


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