Composite

Part:BBa_K539742:Experience

Designed by: Yang, Yi-Pei   Group: iGEM11_NCTU_Formosa   (2011-09-22)
Revision as of 17:01, 5 October 2011 by Zcc (Talk | contribs) (→‎Experience by 2011 iGEM NCTU_FORMOSA)

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Applications of BBa_K539742

Experience by 2011 iGEM NCTU_FORMOSA

Expression analysis in different host cell

We transform the circuit Part:BBa_K539742 ,which is inserted in pSB4A5, into DH5α and EPI300 and collect the bacteria incubated for 0HR, 24HR and 40 HR to find out the most appropriate host cell. After the analysis of GC we find out that DH5α is much better than EPI300 for this circuit, because it can provide higher productive rate of isobutanol. Therefore, we chose DH5α as the host in the following experiments.

Butanol-10.1.png

Figure 10. This diagram indicates that DH5α is very suitable for this circuit Part:BBa_K539742 , because it can provide higher productive rate of isobutanol.

User Reviews

UNIQ7ce5a39fd14bc632-partinfo-00000000-QINU UNIQ7ce5a39fd14bc632-partinfo-00000001-QINU