Device

Part:BBa_K533002:Experience

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)
Revision as of 15:16, 5 October 2011 by Wishyx (Talk | contribs) (→‎Applications of BBa_K533002)

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Please enter how you used this part and how it worked out.

Applications of BBa_K533002

This part is used for specific binding to proteins with multi-proline peptide in the media and then releasing the cargo after interaction with HIV protease.

E. coli with the plasmid for OmpA-HIV site-SH3 is induced with 0.1mM IPTG at 18 centigrade for 12 hours before harvesting by mild centrifuge.

E. coli expressing OmpA-HIV protease site-SH3 was mixed with mCherry solution at room temperature for 30 minutes.

After washing with distilled water twice, some of the bacteria were fixed on a slip and stained with DAPI.

Color Channel E. coli with IPTG induction E. coli without induction
mCherry Thuexp bind p.png Thuexp bind n.png
DAPI Thuexp bind p d.png Thuexp bind n d.png
merge Thuexp bind p m.png Thuexp bind n m.png

The remaining were mixed with E. coli expressing OmpA-HIV protease at room temperature for 30 minutes to release the cargo.

After washing with distilled water twice, bacteria were fixed on a slip and stained with DAPI.


Color Channel + E. coli expressing protease + E. coli without protease
mCherry Thuexp release p.png Thuexp release n.png
DAPI Thuexp release p d.png Thuexp release n d.png
merge Thuexp release p m.png Thuexp release n m.png

User Reviews

UNIQ6abba58fb4041832-partinfo-00000000-QINU UNIQ6abba58fb4041832-partinfo-00000001-QINU