Coding
HIV-1

Part:BBa_I712667:Experience

Designed by: Rok Gaber   Group: iGEM07_Ljubljana   (2007-10-24)
Revision as of 14:28, 5 October 2011 by Wishyx (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I712667

User Reviews

UNIQ627338ebedf8a872-partinfo-00000000-QINU

No review score entered. Yunxiao

We fused this protease to the C-terminal of OmpA and tested its cutting efficiency with a membrane protein containing its cleavage site.

We induced OmpA-protease fusion protein with IPTG concentration of 0.1mM, 0.5mM and 1mM. After 12hours induction at 18 centigrade, protease activity were observed in all the samples.


;

UNIQ627338ebedf8a872-partinfo-00000002-QINU