Coding
HIV-1

Part:BBa_I712667:Experience

Designed by: Rok Gaber   Group: iGEM07_Ljubljana   (2007-10-24)
Revision as of 14:28, 5 October 2011 by Wishyx (Talk | contribs) (→‎User Reviews)

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Applications of BBa_I712667

User Reviews

UNIQ9903de46f9b10496-partinfo-00000000-QINU

No review score entered. Yunxiao

We fused this protease to the C-terminal of OmpA and tested its cutting efficiency with a membrane protein containing its cleavage site.

We induced OmpA-protease fusion protein with IPTG concentration of 0.1mM, 0.5mM and 1mM. After 12hours induction at 18 centigrade, protease activity were observed in all the samples.


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UNIQ9903de46f9b10496-partinfo-00000002-QINU