Device

Part:BBa_K598014

Designed by: Xiaowei YAN, Tong MU   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 14:08, 5 October 2011 by Xiaowei (Talk | contribs)

pBAD+HH+CdIntron+E0040+b0015


This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.

It is the positive control of the theophylline inducing experiment. For further information, please see ，BBa_K598013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1408


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//plasmidbackbone/copynumber/high
Parameters
emission509nm
excitation470nm
n/apBAD+HH+CdIntron+E0040+b0015
resistancechloramphenicol