Device

Part:BBa_K533003:Experience

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)
Revision as of 14:04, 5 October 2011 by Wishyx (Talk | contribs) (→‎Applications of BBa_K533003)

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Applications of BBa_K533003

Like BBa_I712667, it can specifically cleave proteins in the peptide. The difference is that it is expressed as a membrane protein, which enables it to cut target proteins in the media.

We tested function of the protease with OmpA-HIV protese site-SH3, our part BBa_K533002.

E. coli expressing OmpA-HIV protease site-SH3 were first mixed up in multi-proline mCherry, to give it the red fluorescence marker. After washing with distilled water twice, the remaining bacteria were mixed with E. coli expressing OmpA-HIV protease at room temperature for 30 minutes to release the cargo.

After washing with distilled water twice, bacteria were fixed on a slip and stained with DAPI.


Color Channel + E. coli expressing protease + E. coli without protease
mCherry Thuexp release p.png Thuexp release n.png
DAPI Thuexp release p d.png Thuexp release n d.png
merge Thuexp release p m.png Thuexp release n m.png

User Reviews

UNIQ9f4808571efe498c-partinfo-00000000-QINU UNIQ9f4808571efe498c-partinfo-00000001-QINU