Coding

Part:BBa_K624001

Designed by: SHIH- HAO CHEN   Group: iGEM11_NYMU-Taipei   (2011-09-30)
Revision as of 14:00, 5 October 2011 by Ytt1790 (Talk | contribs)

tetR+RBS+GFP+ter

Add a promoter and a RBS on the upstream will express tetR protein and GFP.

GFP can help detecting tetR expression.

30.JPG31.JPG32.JPG

These are measurements of construct tetR+RBS+GFP under fluorescence microscope. The left, above graph is control group of E.coli strain DH5a. The right, above is tetR+RBS+GFP in plasmid c3 in E.coli , while the below is plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proved the sequence length right, and further sequencing work is under operation.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1358


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Categories
Parameters
None