Device
IR-3-GFP

Part:BBa_K658017:Experience

Designed by: Xin Wu   Group: iGEM11_XMU-China   (2011-10-02)
Revision as of 13:58, 5 October 2011 by Xmuwendy (Talk | contribs) (Applications of BBa_K658017)

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Applications of BBa_K658017

The lux pR strength testing device BBa_K658017 with other lux pR strength testing devices BBa_K658016 BBa_K658018 BBa_K658019 was first cloned into plasmid pSB1A2 respectively, followed by transformation into E.coli strain BL21. Fluorescence was measured when cell growth reached a steady state (around 20h). The results are shown in following figures:

Figure 4 Promoters strength relative to lux pR-3 (BBa_K658006).
Figure 5 Efficiency of promoter lux pR (BBa_R0062) and its three mutants.
Figure 6: Fluorescence of four IR-GFP devices at 20h. 5, 3, 3/5, N represent for IR-3-GFP (BBa_K658017), IR-5-GFP (BBa_K658018), IR-3/5-GFP (BBa_K658019) and IR-GFP (BBa_K658016) respectively.

User Reviews

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