Generator

Part:BBa_K649101

Designed by: Hiroki Yoshise   Group: iGEM11_Tokyo_Tech   (2011-09-23)
Revision as of 13:38, 5 October 2011 by Yosei (Talk | contribs)

PlsrR-lsrR

LsrR is the repressor of the lsr operon and itself. Auto Inducer 2(AI-2) is phosphorylated by LsrK, and phospho AI-2 relieves LsrR repression.

This part contains lsrR and lsrK gene, downstream of lsrR promoter. lsrR promoter and lsrR coding gene in this part work correctly, but lsrK has flame shift mutation and does not work properly.

To confirm LsrR represses lsrA promoter, we constructed BBa_K649105. If you want to see detailed infomation, please click here.

For more information, see our work in [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. Tokyo_Tech 2011 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1725
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1399
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None