Device

Part:BBa_K533001:Experience

Designed by: ZHANG Yunxiao   Group: iGEM11_Tsinghua   (2011-09-29)
Revision as of 13:32, 5 October 2011 by Wishyx (Talk | contribs) (→‎Applications of BBa_K533001)

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Applications of BBa_K533001

When this fusion protein is expressed in E. coli, it will enable specific binding to proteins with a multi-proline peptide.

We tested interaction between OmpA-HIV protease site-SH3 and multi-proline mCherry. The result is also applicable to this part with OmpA and SH3 only.

E. coli with the plasmid for OmpA-HIV site-SH3 is induced with 0.5mM IPTG at 18 centigrade for 12 hours before harvesting by mild centrifuge.

E. coli expressing OmpA-HIV protease site-SH3 was mixed with mCherry solution at room temperature for 30 minutes.

After washing with distilled water twice, some of the bacteria were fixed on a slip and stained with DAPI.

Color Channel E. coli with IPTG induction E. coli without induction
mCherry Thuexp bind p.png Thuexp bind n.png
DAPI Thuexp bind p d.png Thuexp bind n d.png
merge Thuexp bind p m.png Thuexp bind n m.png

User Reviews

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