Coding
Part:BBa_K519010
Designed by: Kotone Miyake Group: iGEM11_Tokyo-NoKoGen (2011-09-30)
SmtA
SmtA is a metallothionein that can bind to heavy metal ions such as Cd(II). This SmtA is cloned from Synechococcus sp. PCC7942, a cyanobacterial strain. SmtA is a type II metallothionein with a reduced cys content.It was reported that marine cyanobacterium that does not originally harbor a homologous gene, when genetically engineered to express SmtA, could tolerate medium containing Cd(II), Zn(II) and Cu(II) (Sode et al., 1998). Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 93
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 93
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 93
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 93
Illegal AgeI site found at 121 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//binding/metal
//proteindomain
//proteindomain
function | |
n/a | SmtA |