Translational_Unit

Part:BBa_K567016:Experience

Designed by: You Wang   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)
Revision as of 08:11, 5 October 2011 by Cathy zks (Talk | contribs) (→‎Applications of BBa_K567016)

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Construction of BBa_K567016

metY-CGA: We have cloned operon metY containing tRNAMet from E.coli to pACYC184. The anticodon of tRNAMet was mutated to TCG (base pairing codon CGA).


Characterization of BBa_K567016

When this part, MetRS PT7-metGN (BBa_K567015)(or MetRS PT7-metGM (BBa_K567014)) and KanaR (BBa_K567020) are co-transformed into the cell, the cell is expected to survive Kana.

fig. Growth of ER2566 with a. metGN + metY-CGA, b. metGM + metY-CGA, c. + metGN, d. + metGM. Growth medium (left): LB Kana+Tet. Growth medium (right): LB Kana.

Cell growth shows that the cells show Kana resistance only when both modified MetRS (metGN) and modified tRNAMet(metY-CGA) are transformed into the cell, proving that metGN works well.


For more information concerning this part, please see [http://2011.igem.org/Team:SJTU-BioX-Shanghai 2011 SJTU-BioX-iGEM]

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