Translational_Unit

Part:BBa_K567016:Experience

Designed by: You Wang   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)
Revision as of 08:11, 5 October 2011 by Cathy zks (Talk | contribs) (→‎Applications of BBa_K567016)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Construction of BBa_K567016

metY-CGA: We have cloned operon metY containing tRNAMet from E.coli to pACYC184. The anticodon of tRNAMet was mutated to TCG (base pairing codon CGA).


Characterization of BBa_K567016

When this part, MetRS PT7-metGN (BBa_K567015)(or MetRS PT7-metGM (BBa_K567014)) and KanaR (BBa_K567020) are co-transformed into the cell, the cell is expected to survive Kana.

fig. Growth of ER2566 with a. metGN + metY-CGA, b. metGM + metY-CGA, c. + metGN, d. + metGM. Growth medium (left): LB Kana+Tet. Growth medium (right): LB Kana.

Cell growth shows that the cells show Kana resistance only when both modified MetRS (metGN) and modified tRNAMet(metY-CGA) are transformed into the cell, proving that metGN works well.


For more information concerning this part, please see [http://2011.igem.org/Team:SJTU-BioX-Shanghai 2011 SJTU-BioX-iGEM]

User Reviews

UNIQ41c267a2d7b1b160-partinfo-00000000-QINU UNIQ41c267a2d7b1b160-partinfo-00000001-QINU