Device

Part:BBa_K598027:Design

Designed by: Tong MU, Shuobing CHEN   Group: iGEM11_Peking_R   (2011-09-28)
Revision as of 07:56, 5 October 2011 by Mutong (Talk | contribs) (→‎Source)

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AND gate (BBa_K228258) regulated by theophylline riboswitch P1G1 (BBa_K598005)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2144
    Illegal BamHI site found at 1214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1976
    Illegal AgeI site found at 1320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1323


Design Notes

We have altered the RBS in the riboswitch, producing a similar gate.


Source

The AND gate we utilized was designed by Anderson and his colleagues and consequently standardly redesigned by PKU_Beijing 09 team.

References