RNA
Part:BBa_K581001:Design
Designed by: Chen Yiwei Group: iGEM11_Peking_Signaling (2011-09-26)
ptsG1-GFP (ptsG1 5' UTR fused with gfp)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 747
Design Notes
This part is designed as the C85G mutant of the ptsG(wt)'s 5' untranslated region fused with gfp.
Teppei Morita et.al’ s work suggests that two mutations (C85G and C87G) in ptsG mRNA could completely impair the ability of SgrS to downregulate its expression, while compensatory mutations of SgrS (G178C and G176C) restore the gene silencing ability. Since a specific repression effect is favored in our comparator device, the characteristics of the conjugate mutated ptsG/SgrS system meet the need quite well.
Also, such a site mutation is designed for biological orthogonality.
Source
Escherichia coli genome.