RNA

Part:BBa_K581000:Design

Designed by: Chen Yiwei   Group: iGEM11_Peking_Signaling   (2011-09-17)
Revision as of 02:38, 5 October 2011 by QINXIAO (Talk | contribs) (→‎Design Notes)

sgrS1+Terminator (small RNA regulator, conjugate part of ptsG1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the G178C mutant of the small RNA SgrS(wt).

Teppei Morita et.al’ s work suggests that two mutations (C85G and C87G) in ptsG mRNA could completely impair the ability of SgrS to downregulate its expression, while compensatory mutations of SgrS (G178C and G176C) restore the gene silencing ability. Since a specific repression effect is favored in our comparator device, the characteristics of the conjugate mutated ptsG/SgrS system meet the need quite well.

Also, such a site mutation is designed for biological orthogonality.

Source

E.coli K-12 strain.

References