RNA

Part:BBa_K581000:Design

Designed by: Chen Yiwei   Group: iGEM11_Peking_Signaling   (2011-09-17)
Revision as of 02:23, 5 October 2011 by QINXIAO (Talk | contribs) (→‎Source)

sgrS1+Terminator (small RNA regulator, conjugate part of ptsG1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site specifically mutated for biological orthogonality. Besides, the design of G178C site mutation is also aimed at restoring its repression effection against C87G mutant ptsG mRNA.

Source

E.coli K-12 strain.

References