Composite

Part:BBa_K649105

Designed by: Hiroki Yoshise   Group: iGEM11_Tokyo_Tech   (2011-09-26)
Revision as of 00:32, 5 October 2011 by Takuya 1613 (Talk | contribs)

PlsrA-gfp-PlsrR-lsrR


Fluorescence intensity is decreased by LsrR repression.
This work is done by Hiroki Yoshise.

This part is described as PlsrA-gfp-PlsrR-lsrR because lsrK of BBa_649101 has mutation and does not work properly.

We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a lsrR gene downstream of lsrA promoter-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. This result shows that LsrR successfully repressed promoter lsrA. The working part we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.


For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. our work in Tokyo_Tech 2011 wiki].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2253
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 770


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