Part:BBa_K539691:Experience

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Applications of BBa_K539691

Figure.1 Firstly , we ligase B0030 with ilvC.

Figure.2 At the sametime , we ligase B0030 with ilvD.

Figure.3 Meanwhile , we ligase B0032 with Alss.

Figure.4 To make sure we obtain the correct part , colony PCR is needed.

Figure.5 Secondly , we ligase Plac with B0032+Alss

Figure.6 Thirdly , we ligase Plac+B0032+Alss with B0030+ilvC

Figure.7 At the same time , we ligase B0030+ilvD with RNA thermometer+tetR+double terminator

Figure.8 Finally , we ligase the whole circuit:promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator

To make sure our design could work , we cotransform BBa_K539691 and BBa_K539742 into DH5α, so the expression of alss, ilvC, ilvD and kivd are under the control of temperature.

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UNIQ5cf3f22607256698-partinfo-00000000-QINU UNIQ5cf3f22607256698-partinfo-00000001-QINU