Reporter

Part:BBa_K598000

Designed by: Yangyang ZHAO, Yan GONG, Zhenrun ZHANG, Qingyang XIAO   Group: iGEM11_Peking_R   (2011-09-18)
Revision as of 14:14, 4 October 2011 by Grasscliff (Talk | contribs)

TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015

This part is TPP up-regulated hammerhead ribozyme, consisting of 131 nucleotides.


Dear,all.

    This is Zhang Mengqi. And now, I will tell you how to design the simplest page on iGEM. Please follow me to see the examples:
     1. you can see this position in that page and the sentence 'This part is TPP up-regulated hammerhead ribozyme, consisting of 131 nucleotides.' here. remember this position and edit within it. Please do not change other parts in this editing page.
     

Remember, you can type your descriptions straightly on this page.

     2.there times of the symbol   (') means make words strong.
     3.type twice the symbol(')means make words lean
     4.please distinct (')and("), and the latter means"what you said"
     5.For more information,please visit:   http://2010.igem.org/Team:Peking/Parts
     6.How to send a picture here?
       6.1 the first thing you should know is how to send picture to iGEM's Internet:
          Open any page of 2011 iGEM, and find the link"upload a file"at the bottom, get in, and you can see the web like picture 3. please remember that never forget to name the picture with the beginning"PekingR Parts"

or your picture may be upload by others who don't know that this picture is named(everyone has the right to upload the file, so be careful not let your pictures been uploaded by others)

          when uploaded,you can see the screen like picture 5, then click the time,and get the link of the picture.
      6.2  type the word like this following 2 in the editing page:
           

PekingR_team_smallbanner2.3.png

      6.3 you can also make links of your picuture by ding this:

       So, that's all you need about the picture.
     Finally, save page and you can see the change.There can be something worng for the users of chrome or safari, don't worry.
     Good luck!


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 121
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 794


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//plasmidbackbone/copynumber/high
//regulation/negative
Parameters
emission509nm
excitation470nm
ligandsThiamine Pyrophosphate (TPP)
n/aTPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015
resistance chloramphenicol