Signalling

Part:BBa_K574005:Design

Designed by: Lei Wei   Group: iGEM11_Tsinghua-A   (2011-09-29)
Revision as of 14:04, 4 October 2011 by Destiny (Talk | contribs) (→‎Design Notes)

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3OC12HSL and YFP regulated by pBad


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 820
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 382
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design this part to increase the 3OC12HSL in the environment on our demands. It is also designed to achieve our goals to regulate the cycle of our Oscillator.

Source

We used BioBrick Standard Assembly. List as follows: BBa_I13453 BBa_K081009 BBa_E0430

References