Regulatory
recAp

Part:BBa_K629001:Design

Designed by: Zilong Wang, Yi ZHENG   Group: iGEM11_SYSU-China   (2011-10-03)
Revision as of 13:48, 4 October 2011 by Richard90 (Talk | contribs) (→‎References)

recAp, could be started with exposure to irradiation, UV, nalidixic acid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the -35 and -10 district, the RBS


Source

E. Coli BL21 plys genome

References

S. NUYTS. et al. Insertion or Deletion of the Cheo Box Modifies Radiation Inducibility of Clostridium Promoters. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 67(10). 4464-4470(2001).

S Nuyts. et al. Radio-responsive recA promoter significantly increases TNFa production in recombinant clostridia after 2 Gy irradiation. Gene Therapy 8. 1197-1201(2001).

Sandra Nuyts. et al. The Use of Radiation-Induced Bacterial Promoters in Anaerobic Conditions: A Means to Control Gene Expression in Clostridium-Mediated Therapy for Cancer. RADIATION RESEARCH 155. 716~723(2001).