Coding
trkD

Part:BBa_K629005:Design

Designed by: Zilong WANG, Yi ZHENG   Group: iGEM11_SYSU-China   (2011-10-04)
Revision as of 13:34, 4 October 2011 by Richard90 (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

trkD, a functional Kup (formerly TrkD) system took up Cs+ with a moderate rate and affinity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1320


Design Notes

none


Source

genome of E. Coli BL21 plys

References

IRK BOSSEMEYER. et al. Specific Cesium Transport via the Escherichia coli Kup (TrkD) K+ Uptake System.JOURNAL OF BACTERIOLOGY 171(4), 2219-2221(1989).

Simon V. Avery. Caesium accumulation by microorganisms: uptake mechanisms, cation competition, compartmentalization and toxicity. Journal of Industrial Microbiology 14. 76-84(1995).

JANE E. LOPILATO. et al.D-Ribose Metabolism in Escherichia coli K-12: Genetics, Regulation, and Transport. JOURNAL OF BACTERIOLOGY 158(2), 665-673(1984).

DONALD C. DOSCH.et al. Genetic Analysis of Potassium Transport Loci in Escherichia coli: Evidence for Three Constitutive Systems Mediating Uptake of Potassium. JOURNAL OF BACTERIOLOGY 173(2). 687-696(1991).

Joy Perkins and Geoffrey M.Gadd. The influence of pH and external K+ concentration on caesium toxicity and accumulation in Escherichia coli and Bacillus subtilis. Journal of Industrial Microbiology 14. 219-225(1995).