RNA

Part:BBa_K581000:Design

Designed by: Chen Yiwei   Group: iGEM11_Peking_Signaling   (2011-09-17)
Revision as of 13:08, 4 October 2011 by QINXIAO (Talk | contribs) (→‎Design Notes)

sgrS1+Terminator (small RNA regulator, conjugate part of ptsG1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Site specifically mutated for biological orthogonality. Besides, the design of G178C site mutation is also aimed at restoring its repression effection against C87G mutant ptsG mRNA.

Source

E.coli K12 strain.

References