Plasmid
Part:BBa_K524005:Design
Designed by: HO Yuan Heng Trevor Group: iGEM11_HKUST-Hong_Kong (2011-10-04)
pToolkit
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1215
Illegal EcoRI site found at 2724
Illegal PstI site found at 2219
Illegal PstI site found at 2466 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1215
Illegal EcoRI site found at 2724
Illegal PstI site found at 2219
Illegal PstI site found at 2466 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1215
Illegal EcoRI site found at 2724
Illegal BamHI site found at 1154 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1215
Illegal EcoRI site found at 2724
Illegal PstI site found at 2219
Illegal PstI site found at 2466 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1215
Illegal EcoRI site found at 2724
Illegal PstI site found at 2219
Illegal PstI site found at 2466
Illegal AgeI site found at 989
Illegal AgeI site found at 2646 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4422
Illegal SapI site found at 971
Design Notes
We construct the pToolkit by replacing its heat sensitive replication origin oriR101&RepA101 with pir gene regulated replication origin ori-gamma.
Source
The backbone of the pToolkit, including the lambda-red swapping system and the Ampicilin Resistance Gene comes from pKD46. The replication origin of the pToolkit is ori-gamma, from the relication origin R6K.