Signalling

Part:BBa_K574004:Design

Designed by: Lei Wei   Group: iGEM11_Tsinghua-A   (2011-09-29)
Revision as of 04:39, 4 October 2011 by Destiny (Talk | contribs) (→‎Source)

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3OC12HSL regulated by TetR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 744
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 306
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design this part to regulate the sending of 3OC12HSL. TetR can act as a represser, and aTc can be regarded as an activator when TetR exists.

Source

We used BioBrick Standard Assembly. List as follows: BBa_R0040 BBa_K081016

References