Coding
Bcr
Part:BBa_K524100:Design
Designed by: Shek Chin Hong Group: iGEM11_HKUST-Hong_Kong (2011-09-27)
Bcr - multidrug efflux system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 503
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 99
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
'GUG' start codon has been mutated to 'AUG'. Stop codons at the end of the coding sequence have been changed to 'TAATAA'.
Source
Amplified from genomic DNA of Escherichia coli K-12 Strain RR1
References
Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism [http://ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG11419]
Kunihiko Nishino, Eiji Nikaido, Akihito Yamaguchi, Regulation and physiological function of multidrug efflux pumps in Escherichia coli and Salmonella, Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, Volume 1794, Issue 5, May 2009, Pages 834-843, ISSN 1570-9639, 10.1016/j.bbapap.2009.02.002. [http://www.sciencedirect.com/science/article/pii/S1570963909000260]