Device
IR-GFP
Part:BBa_K658016
Designed by: Yidan Hu Group: iGEM11_XMU-China (2011-10-02)
LuxI->LuxR->lux pR->GFP
This device is made up of three parts: a LuxI producer, a Lux R producer and a GFP reporter. It is used to test the efficiency of promoter lux pR(BBa_R0062) and its mutants lux pR-3 (BBa_K658006), lux pR-5 (BBa_K658007), lux pR-3/5 (BBa_K658008).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1874
Illegal BsaI.rc site found at 2601
[edit]
Categories
Parameters
control | R0011 |
proteins | LuxI LuxR GFP |
signalling_molecule | AHL |