Generator
Part:BBa_K524004
Designed by: HO Yuan Heng Trevor Group: iGEM11_HKUST-Hong_Kong (2011-10-03)
pir gene
The pir gene codes for the pi protein. Initiation of replication at ori-gamma requires the pi protein in trans. Doubling the concentration of pi protein would, however, effectively shut down the replication. Expression of pi protein is autogenously regulated.
Usage and Biology
The presence of pir gene can allow maintenance of plasmids with R6K origins as their sole origin of replication.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 438
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |