Composite

Part:BBa_K649200

Designed by: SeoHyun Kim   Group: iGEM11_Tokyo_Tech   (2011-09-18)
Revision as of 10:35, 3 October 2011 by Carakim01 (Talk | contribs)

PlacIQ-lox2272-gfp-lox2272

lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied by both in vitro and in vivo assay, and proved to be working.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 107
    Illegal BamHI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 790


[edit]
Categories
Parameters
n/aPlacIQ-lox2272-gfp-lox2272