Signalling

Part:BBa_K574009:Design

Designed by: Lei Wei   Group: iGEM11_Tsinghua-A   (2011-09-29)
Revision as of 08:48, 3 October 2011 by Destiny (Talk | contribs) (→‎Design Notes)

3OC12HSL -> PoPS Receiver


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1048
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 386
    Illegal AgeI site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to design a system whose input is 3OC12HSL, then we combine LasR coding sequence(BBa_C0079) with relevant promoter(BBa_R0079). Considering the possibility for various designs in the future(or in other words, the expandability), those regulated by Plas promoter depends on your need.

Source

References