Device

Part:BBa_K598009:Design

Designed by: Siyang HAO, Zhenrun ZHANG   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 07:04, 3 October 2011 by Hsy (Talk | contribs) (→‎Source)

pBAD+Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 869


Design Notes

Change the promoter if required.


Source

BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive promoter library. RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation.

References

Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.