Intermediate

Part:BBa_K526000:Design

Designed by: Sung Kuk Lee   Group: iGEM11_UNIST_Korea   (2011-09-26)
Revision as of 06:51, 3 October 2011 by Skleelab (Talk | contribs) (→‎Source)

Pompc promoter coding fimE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 750
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 750
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 750
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 750
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no special design consideration. We can amplify the Pompc and fimE from E. coli genomic DNA and assembly them using Gibson's assembly.

Source

Both fimE and Pompc was amplified from E. coli genomic DNA.

References