Composite
Part:BBa_K557002
Designed by: Yinghong Lan Group: iGEM11_USTC-China (2011-10-02)
Toggle-switch+Aptamer+CheZ
This part is the assembly of riboswitches followed by an important gene CheZ in keeping E.coli motile (BBa_K557001) finely tuned by small molecules and toggle switches (BBa_K228003) pushed on and off while memorizing current state. It would make it possible that bacterial colonies will automatically divide into two parts after certain time, during which riboswitches would act as the main power to drive two parts away from each other while toggle switches would play the role of giving birth to two 'different' kinds of bacteria in one colony.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 10
Illegal AgeI site found at 122 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
function | allow bacterial colonies to automatically divide into two parts after certain time |