Composite

Part:BBa_K649105

Designed by: Hiroki Yoshise   Group: iGEM11_Tokyo_Tech   (2011-09-26)
Revision as of 09:31, 2 October 2011 by Yosei (Talk | contribs)

PlsrA-gfp-PlsrR-lsrR

Median fluorescence intensity(MFI) of BBa_K649104(PlsrA-RBS-gfp) is 3-fold higher than that of BBa_K649105(PlsrA-gfp-PlsrR-lsrR).

After four hours from OD590 reaching 0.15, we measured OD.
This work is done by Hiroki Yoshise.
Median fluorescence intensity(MFI) is decreased by LsrR repression.
This work is done by Hiroki Yoshise.


We confirmed that LsrR represses lsrA promoter.

For this purpose, we constructed BBa_K649105(PlsrA-gfp-PlsrR-lsrR) and compared fluorescence intensity levels of BBa_K649104(PlsrA-RBS-gfp) and BBa_K649105.

For more information, see our work in Tokyo_Tech 2011 wiki


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2579
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2253
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 770


[edit]
Categories
Parameters
None