Generator

Part:BBa_K624018

Designed by: Shang-Jui Tsai   Group: iGEM11_NYMU-Taipei   (2011-10-02)
Revision as of 08:25, 2 October 2011 by Registry (Talk | contribs)

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pYMB essentials+[YN-13-R]+[YC-CHAMP]

pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, YN-13-R and YC-CHAMP is ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode. BiFC between YN and YC is anticipated in the default state as well.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal NgoMIV site found at 1824
    Illegal NgoMIV site found at 1908
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2577


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Parameters
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