Plasmid
APEV

Part:BBa_K596004:Design

Designed by: Ebuzer Kalyoncu   Group: iGEM11_Bilkent_UNAM_Turkey   (2011-09-19)
Revision as of 08:09, 2 October 2011 by Rezube (Talk | contribs) (→‎Design Notes)

Algae Protein Expression Vector


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3787
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3787
    Illegal NheI site found at 3376
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 3793
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3787
    Illegal BamHI site found at 23
    Illegal XhoI site found at 3779
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3787
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3787
    Illegal XbaI site found at 3802
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2598
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1621
    Illegal SapI site found at 538


Design Notes

This part contains EcoRI,XhoI,SpeI and PstI restriction sites for biobrick standard.

Source

a

References